Job i Odense
The Department of Biochemistry and Molecular Biology at the University of Southern Denmark invite applications for a postdoctoral research fellowship (two years) in the area of fundamental mass spectrometry/proteomics.
Current proteomics approaches lack complete protein coverage, largely due to the almost exclusive use of positive ion mode as the ionization method, which results in inefficient ionization of many acidic peptides. With the existence of equal amount of acidic and alkaline proteins (the same distribution for their derived peptides) in the human proteome, this inefficient ionization poses a significant challenge, yet at the same time presents substantial potential. This fact underlines the timeliness to advance the area of proteomics by making use of the largely unexplored negative ion mode.
The successful applicant will be responsible for investigating settings of liquid chromatography and mass spectrometry ideal for detection of negatively charged peptides. This includes systematic testing of mobile phases, column materials, proteolytic enzymes, and MS parameters. You will be using this setup to characterize and validate a number of peptide-specific features, e.g. peptide retention times, and develop in collaboration machine-learning and statistical tools that ensures reliable protein identification and quantification. The developed methodology will be tested in...